Manufactured / Disease models
ISOGENIC CELL LINESDisease models
Isogenic human cell lines carrying a single pathogenic variant, edited at the endogenous locus, so the only difference from the parent line is the one that causes the disease.
- Isogenic
- Matched to the parent
- Endogenous
- Edited at the native locus
- Verified
- Sequence, STR, mycoplasma
Coming soon
2 new disease model cell lines
Isogenic human cell lines carrying a single pathogenic variant, edited at the endogenous locus. No selection cassette, no silent passenger mutations, no residual recombination scars. Each line differs from its parent at the disease-causing base and nowhere else.
CFTR c.1624G>T (p.Gly542Ter, G542X)
in 16HBE14o- (Immortalised Human Bronchial Epithelium)
Class I nonsense variant in a bronchial epithelial background with native CFTR expression. For readthrough compounds, nonsense-mediated decay studies and gene correction work.
ASS1 c.1168G>A (p.Gly390Arg)
in Huh7 (Human Hepatoma)
Citrullinaemia type I, hepatocyte-derived background. Supplied alongside an ASS1 knockout line from the same editing campaign if a complete loss of function comparator is useful.
Quality
What ships with every line
Custom
Custom disease models, built to order
Point mutations, deletions, insertions and knockouts in the background your assay already runs in. Send us the gene, the variant in HGVS notation and the parental line, and we will come back on feasibility and timing.
The gene
The gene you want to model.
The variant
In HGVS notation, for example c.1624G>T.
The parental line
The background your assay already runs in.
Feasibility and timing
We come back to you on both.
Tell us the gene and the variant
Gene, variant in HGVS notation and parental line. We will come back on feasibility and timing.